Interactions Between High Load of a Bio-based and Biodegradable Plastic and Nitrogen Fertilizer Affect Plant Biomass and Health: A Case Study with Fusarium solani and Mung Bean (Vigna radiata L.)

Abstract Bio-based and biodegradable plastics such as mulching films are widely used in agricultural field sites. However, there limited studies of their impact on plant development health even though an important soil-borne pathogen F . solani has been reported to associate with various types bio-based plastics, especially polybutylene succinate-co-adipate (PBSA). To evaluate the influence PBSA amendment soils health, F. mung bean ( V. radiata ) were models a modified petri-dish test using soil suspensions. Mung seeds incubated suspensions two dilutions (high vs. low dilution high amendment) pre-incubated 1 year under different treatments (combinations N fertilizer (ammonium sulfate) load) petri dish Plant disease incidence recorded both microscopic molecular techniques (specific PCR Illumina amplicon sequencing). Treatment non-sterile strongly increased caused by V at dilution. At dilution, incident was 67.5% while reached 92.5%. In contrast, but without fertilizer, non observed. Apart from infection, other fungi can also infect seedlings, levels. Nevertheless, based this short-term study, we found no evidence that alone significantly increase overall incidence.